Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US10266889B2 · kind B2 · utility
5Cited by
3References
3Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Jul 3, 2013 |
| Grant date | Apr 23, 2019 |
| Priority date | — |
| Expiry date | Jul 3, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to modified oligonucleotide compositions and methods for selectively reducing unwanted nucleic acid contaminants and enriching for desired nucleic acid targets from complex genomic nucleic acid mixtures for sequencing applications. The modified oligonucleotide compositions include one or more modified groups that increase the Tm of the resultant oligonucleotide composition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.