Method for detection of a reversibly photo-convertible fluorescent species
US10267732B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 21, 2014 |
| Grant date | Apr 23, 2019 |
| Priority date | — |
| Expiry date | Feb 11, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detection of at least one reversibly photo-convertible fluorescent species, comprises the following steps: a) illumination of a sample comprising said or at least one of the reversibly photo-convertible fluorescent species by a periodically modulated illuminating light; and b) detection of fluorescent emission emitted by the sample thus illuminated; wherein the method further comprises the following step: c) extraction of the amplitude of the intensity component of the fluorescent emission exhibiting the same periodicity as the periodically modulated illuminating light and a phase quadrature with respect to the same; and wherein the mean intensity of the illuminating light and the modulation frequency of the same are chosen to maximize the amplitude of the intensity component of the fluorescent emission.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.