Methods and devices based upon a novel form of nucleic acid duplex on a surface

US10272409B2 · kind B2 · utility

Inventors

• Michael E. Hogan · Princeton, US
• Sergy Lemeshko · Houston, US
• Yuri Belosludtsev · Tucson, US
• Thomas F. Powdrill · College Station, US
• Rahul Mitra · Houston, US
• Joseph G. Utermohlen · Tucson, US
• Frederick Henry Eggers · Sahuarita, US

Key dates

• Filing date: Apr 4, 2008
• Grant date: Apr 30, 2019
• Expiry date: Apr 4, 2028

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2219/00722
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.