Enzymes for producing non-straight-chain fatty acids

US10273511B2 · kind B2 · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• Rachelle A. S. Lemke · Madison, US
• Timothy James Donohue · Menlo Park, US
• Joshua J. Coon · Charlottesville, US
• Amelia C. Peterson · Bremen, DE
• Michael S. Westphall · Fitchburg, US

Key dates

• Filing date: Jun 30, 2015
• Grant date: Apr 30, 2019
• Expiry date: Jun 30, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P17/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzymes for producing non-straight-chain fatty acids, microorganisms comprising the enzymes, and in vivo and in vitro uses of the enzymes. Provided are enzymes capable of producing various non-straight-chain fatty acids, including branched-chain fatty acids, cyclic fatty acids, and furan-containing fatty acids. The enzymes include RSP2144, RSP1091, and RSP1090 from Rhodobacter sphaeroides and homologs thereof. The enzymes can be purified to produce non-straight-chain fatty acids in vitro or expressed in microorganisms to produce non-straight-chain fatty acids in vivo. The microorganisms can be fine-tuned to produce a specific type of non-straight-chain fatty acid by expressing, overexpressing, or deleting the enzymes in various combinations.