Patent · US Active

Error-free sequencing of DNA

US10273538B2 · kind B2 · utility

0Cited by

1References

12Claims

0Family size

Assignee

Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

Christoph Klein · Darmstadt, DE
Stefan Kirsch · Heidesheim, DE
Zbigniew Tadeusz Czyz · Regensburg, DE
Urs Lahrmann · Regensburg, DE

Key dates

Filing date	Feb 5, 2015
Grant date	Apr 30, 2019
Priority date	—
Expiry date	Oct 1, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a novel method of error-free sequencing of DNA. Further, the present invention provides for a four-part oligonucleotide, comprising a fixed sequence, a randomized sequence, a restriction nuclease recognition site and/or restriction site, and a primer binding site. The invention also relates to the use of the sequenced DNA fragments obtained by the methods of the invention in methods for DNA sequence analysis, generation of cell lineage trees or assessment of copy numbers.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.