Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US10273540B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 2013 |
| Grant date | Apr 30, 2019 |
| Priority date | — |
| Expiry date | Feb 27, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B99/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.