Heparin affinity tag and application thereof

US10280196B2 · kind B2 · utility

Assignee

• THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS · US

Inventors

• Suresh Kumar Thallapuranam · Fayetteville, US
• Srinivas Jayanthi · Fayetteville, US
• Jacqueline Morris · Fayetteville, US
• Alicia Brown · Bellflower, US
• David S. McNabb · Fayetteville, US
• Ralph Henry · Fayetteville, US

Key dates

• Filing date: Jan 20, 2015
• Grant date: May 7, 2019
• Expiry date: Jul 18, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.