Nucleic acid amplification primers for PCR-based clonality studies

US10280462B2 · kind B2 · utility

Inventors

• Jacobus Johannes Maria van Dongen · Nieuwerkerk aan den IJssel, NL
• Anthonie Willem Langerak · Barendrecht, NL
• Eduardus Maria Dominicus Schuuring · Groningen, NL
• Jesus Fernando San Miguel · Salamanca, ES
• Ramon Garcia Sanz · Salamanca, ES
• Antonio Parreira · Lisbon, PT
• John Lewis Smith · Calne, GB
• Frances Louise Lavender · Ormskirk, GB
• Gareth John Morgan · Leatherhead, GB
• Paul Anthony Stuart Evans · Heysham, GB
• Michael Kneba · Westensee, DE
• Michael Hummel · Berlin, DE
• Elizabeth Anne Macintyre · Meudon, FR
• Christian Bastard · Ardouval, FR
• Frederic Davi · Meudon, FR
• Monika Brüggemann · Kiel, DE

Key dates

• Filing date: Jun 23, 2014
• Grant date: May 7, 2019
• Expiry date: Sep 17, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-Jκ IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.