DHAD variants and methods of screening
US10287566B2 · kind B2 · utility
2Cited by
80References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 16, 2017 |
| Grant date | May 14, 2019 |
| Priority date | — |
| Expiry date | Mar 22, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/52
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.