RNA virus-derived plant expression system

US10287602B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Sylvestre Marillonnet · Bodenwerder, DE
• Carola Engler · Bodenwerder, DE
• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Jan 5, 2016
• Grant date: May 14, 2019
• Expiry date: Sep 18, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.