Activation of bioluminescence by structural complementation

US10288605B2 · kind B2 · utility

Assignee

• Promega Corporation · US

Inventors

• Andrew S. Dixon · Verona, US
• Lance P. Encell · Fitchburg, US
• Mary Hall · Waunakee, US
• Keith V. Wood · Verona, US
• Monika G. Wood · Verona, US
• Marie Schwinn · Madison, US
• Brock Binkowski · Sauk City, US
• Hicham Zegzouti · Madison, US
• Nidhi Nath · Madison, US
• Subhanjan Mondal · Middleton, US
• Said Goueli · Fitchburg, US
• Poncho Meisenheimer · San Luis Obispo, US
• Thomas Kirkland · Atascadero, US
• James Unch · Arroyo Grande, US
• Dileep K. Pulukkunat · Middleton, US
• Matthew Robers · Madison, US
• Melanie Dart · Madison, US
• Thomas Machleidt · Madison, US

Key dates

• Filing date: Jun 29, 2018
• Grant date: May 14, 2019
• Expiry date: Jun 29, 2038

Classification

• Technology area (CPC H): Electricity
• CPC primary: H04W4/38
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.