Patent · US Active

Assay for prions

US10288627B2 · kind B2 · utility

0Cited by

2References

10Claims

0Family size

Assignee

D-GEN LIMITED · GB

Inventors

Graham Jackson · London, GB
John Collinge · London, GB
Julie Ann Edgeworth · London, GB

Key dates

Filing date	Sep 13, 2011
Grant date	May 14, 2019
Priority date	—
Expiry date	Aug 11, 2032

Classification

Technology area (CPC G)Physics
CPC primaryG01N2800/2828
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to a method for detection of abnormal PrP in a sample of blood or urine, said method comprising: (a) diluting the sample with buffer to comprise final concentrations of (i) 10 mM to 500 mM buffer agent; (ii) 1% to 10% w/v bovine serum albumin; and (iii) 1% to 8% w/v CHAPS; (b) adding steel particles and incubating to allow PrP binding; (c) washing the steel particles to remove diluted sample; and (d) detecting abnormal PrP captured on the steel particles using antibody capable of binding said abnormal PrP. The invention also provides compositions and kits.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.