Modified Bacillus cereus phospholipase C protein and method of processing vegetable oil
US10294463B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 19, 2014 |
| Grant date | May 21, 2019 |
| Priority date | — |
| Expiry date | Nov 27, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/04003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A modified Bacillus cereus phospholipase C enzyme is provided, as well as a method of using the modified phospholipase C enzyme in a method of treating vegetable oil. In certain embodiments, this method may comprise combining a vegetable oil with an modified phospholipase C enzyme comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid residue at position 66 is a Trp (W) or Tyr (Y), and maintaining the combination under conditions suitable for the modified phospholipase C enzyme to catalyze the hydrolysis of phospholipids in the oil to produce diacylglycerol and a water soluble phosphate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.