Candida carbonyl reductase and method for preparing (R)-lipoic acid precursor

US10294479B2 · kind B2 · utility

Assignee

• East China University of Science and Technology · CN

Inventors

• Jianhe Xu · Nanjing, CN
• Yujun Zhang · Hamburg, DE
• Gaowei Zheng · Zhejiang, CN
• Jiang Pan · Nanjing, CN

Key dates

• Filing date: Mar 4, 2015
• Grant date: May 21, 2019
• Expiry date: Mar 4, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y101/01184
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.