Methods and compositions involving ALS variants with native substrate preference

US10294487B2 · kind B2 · utility

Assignees

• E.I. DU PONT DE NEMOURS AND COMPANY · US
• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Henrik Albert · Alameda, US
• John Lawrence Andreassi, II · Hockessin, US
• Sean Bertain · Piedmont, US
• Linda A. Castle · Mountain View, US
• Steven Gutteridge · Wilmington, US
• Daniel L. Siehl · Menlo Park, US
• KAY WALTER · Sunnyvale, US

Key dates

• Filing date: Jun 12, 2013
• Grant date: May 21, 2019
• Expiry date: Nov 3, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8278
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.