Mutant endoglycoceramidases with enhanced synthetic activity
US10294504B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 29, 2015 |
| Grant date | May 21, 2019 |
| Priority date | — |
| Expiry date | Jun 7, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01123
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.