Patent · US Active

Sequencing method employing ternary complex destabilization to identify cognate nucleotides

US10294514B2 · kind B2 · utility

10Cited by
37References
42Claims
0Family size

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Key dates

Filing dateApr 28, 2017
Grant dateMay 21, 2019
Priority date—
Expiry dateDec 1, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6874
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.