Identification of nucleic acid template-linked barcodes comprising nucleic acid modifications

US10294523B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Benjamin Flusberg · Atlanta, US
• Jonas Korlach · Camas, US
• Jeffrey Wegener · Cupertino, US
• Tyson A. Clark · Menlo Park, US
• Igor D. Vilfan · East Palo Alto, US
• Andrey Kislyuk · San Ramon, US
• Stephen Turner · Menlo Park, US
• Jon Sorenson · Alameda, US
• Kevin Travers · Menlo Park, US
• Cheryl Heiner · La Honda, US
• Austin B. Tomaney · Burlingame, US
• Patrick Marks · San Francisco, US
• Jessica Lee · Cupertino, US
• Lei Jia · Palo Alto, US
• Dale Webster · San Mateo, US
• John Lyle · Fremont, US
• Jeremiah Hanes · Woodside, US

Key dates

• Filing date: Sep 23, 2015
• Grant date: May 21, 2019
• Expiry date: Jul 3, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.