Use of two-photon excited fluorescence in assays of clinical chemistry analytes
US10295465B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 11, 2005 |
| Grant date | May 21, 2019 |
| Priority date | — |
| Expiry date | May 4, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/204998
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to an in vitro diagnostic method for quantification of a clinical chemistry analyte from a clinical sample wherein the clinical chemistry analyte undergoes a chemical reaction or reactions with a reagent or reagents in one or several steps, or in a reaction sequence, or catalyzes a chemical reaction, or reactions, or a reaction in a reaction sequence of a reagent or reagents, in one or several steps, in a reaction system. The reaction or reactions or reaction sequence result in a change of a measurable property of a compound or compounds of said reaction or reactions or reaction sequence. Characteristic for the method is that said chemical reaction or reactions or reaction sequence results in formation of a two-photon fluorescent compound, or a change in two-photon fluorescence properties of the reaction system comprising at least one two-photon fluorescent compound, and the analyte is quantified by exciting said two-photon fluorescent compound or compounds and measuring two-photon exited fluorescence, and relating said measured fluorescence to method standardization data based on measurements obtained from reference material of said analyte. The present inven…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.