Patent · US Active

Regulatable promoter

US10301634B2 · kind B2 · utility

1Cited by

3References

26Claims

0Family size

Assignee

LONZA LTD · CH

Inventors

Diethard Mattanovich · Wien, AT
Brigitte Gasser · Wien, AT
Michael Maurer · Wien, AT
Roland Prielhofer · Wien, AT
Joachim Klein · Hochspeyer, DE
Jana Wenger · Visp, CH

Key dates

Filing date	Oct 26, 2016
Grant date	May 28, 2019
Priority date	—
Expiry date	Oct 26, 2036

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02E50/10
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.