Method of amplifying circular DNA
US10301672B2 · kind B2 · utility
7Cited by
0References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 18, 2015 |
| Grant date | May 28, 2019 |
| Priority date | — |
| Expiry date | Nov 18, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for easily and exponentially amplifying circular DNA, particularly long chain circular DNA, in a cell-free system. Specifically, the present invention provides a method for amplifying circular DNA in which circular DNA having a replication origin sequence (origin of chromosome (oriC)) is mixed with a reaction solution containing the following enzyme groups to form a reaction mixture, which is then reacted under an isothermal condition, the enzyme groups being:
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.