Methods of preventing and removing trisulfide bonds
US10308706B2 · kind B2 · utility
1Cited by
11References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 4, 2015 |
| Grant date | Jun 4, 2019 |
| Priority date | — |
| Expiry date | Jul 9, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/1133
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.