Hydrolases, nucleic acids encoding them and methods for making and using them

US10308920B2 · kind B2 · utility

Assignee

• DSM IP ASSETS B.V. · NL

Inventors

• Uwe Bornscheuer · Greifswald, DE
• David Weiner · Echt, NL
• Tim Hitchman · Carlsbad, US
• Jonathan Lyon · San Diego, US
• Sirirung Wongsakul · Mae Jo, TH

Key dates

• Filing date: Oct 7, 2015
• Grant date: Jun 4, 2019
• Expiry date: Oct 7, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/02
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, t…