Real-time imaging method of NAPDH-dependent metabolic activity in cell and use thereof
US10308973B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 14, 2018 |
| Grant date | Jun 4, 2019 |
| Priority date | — |
| Expiry date | Sep 14, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y101/01001
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a real-time imaging method of NAPDH-dependent metabolic activity in a cell and a use thereof, and more particularly, an imaging method capable of measuring concentration gradient and change in an intracellular coenzyme using a mutant of metagenome-derived blue fluorescent protein (mBFP) in real-time and an application method thereof. In particular, the method of imaging and quantifying the coenzyme contained in various biological samples may be performed by improving fluorescence in a short time even under anaerobic conditions, without adding a substrate required for coenzyme measurement or conversion without destroying cells, and without consuming the time required for formation of a special structure (fluorophore) for generating fluorescence unlike the existing quantitative systems. In particular, provided is a stable quantitative/imaging technique that is directly applicable to biological samples having various complex compositions without signal interference caused by impurities by removing an inherent enzymatic activity of mBFP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.