Methods of conducting biochemical reactions while reducing reactive molecular species during electrowetting

US10309927B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Rigo Pantoja · San Diego, US
• Rachel SCHOWALTER · San Diego, US
• Jimmy Perrott · San Diego, US
• Cyril Delattre · San Diego, US
• Allen E. Eckhardt · San Diego, US

Key dates

• Filing date: Apr 7, 2016
• Grant date: Jun 4, 2019
• Expiry date: Aug 27, 2037

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0427
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Some embodiments disclosed herein provide methods of reducing reactive molecular species in a reaction carried out in a digital fluidics device having one or more electrodes by using a reaction mixture comprising an electron acceptor additive. In some embodiments, the presence of the electron acceptor additive reduces the presence of reactive molecular species in the reaction mixture. In some embodiments, the presence of the electron acceptor additive reduces the formation of gas bubbles in the digital fluidic device. Therefore, in preferred embodiments, the electron acceptor additive does not form a gas when reduced.