High growth capacity auxotrophic Escherichia coli and methods of use
US10316322B2 · kind B2 · utility
3Cited by
2References
23Claims
0Family size
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Key dates
| Filing date | Jun 30, 2015 |
| Grant date | Jun 11, 2019 |
| Priority date | — |
| Expiry date | Jun 30, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides high growth capacity strains of auxotrophic Escherichia coli and methods for generating thereof. The high growth capacity strains express a complementing auxotrophic plasmid that allows the strain to grow in the absence of the auxotrophic amino acid. Also, provided herein is a method for preparing a bacterial cell extract of a high growth capacity strain of auxotrophic Escherichia coli for use in an in vitro protein expression.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.