Method of constructing sequencing library with bubble-shaped adaptor element

US10316356B1 · kind B1 · utility

Assignee

• MGI Tech Co., Ltd. · CN

Inventors

• Yuan Jiang · Richland, US
• Kai Tian · Hefei, CN
• Xia Zhao · Highland Park, US
• Wenwei Zhang · Longbeilingcun, CN
• Huaiqian Xu · Nanhu, CN
• Hui Jiang · Lo Wu, CN
• Radoje Drmanac · Los Altos Hills, US
• Chunyu Geng · Nanhu, CN

Key dates

• Filing date: Nov 21, 2014
• Grant date: Jun 11, 2019
• Expiry date: May 22, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2535/122
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3′-terminal and the 5′-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3′-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.