Oscillating amplification reaction for nucleic acids
US10316358B2 · kind B2 · utility
9Cited by
35References
47Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 25, 2016 |
| Grant date | Jun 11, 2019 |
| Priority date | — |
| Expiry date | Feb 21, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.