Molecular detection of enterovirus and parechovirus
US10316372B2 · kind B2 · utility
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Key dates
| Filing date | Sep 7, 2018 |
| Grant date | Jun 11, 2019 |
| Priority date | — |
| Expiry date | Sep 7, 2038 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.
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