Protein retention expansion microscopy
US10317321B2 · kind B2 · utility
49Cited by
4References
27Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 5, 2016 |
| Grant date | Jun 11, 2019 |
| Priority date | — |
| Expiry date | Apr 19, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/95
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method termed protein retention ExM (proExM), in which proteins, rather than labels, are anchored to the swellable gel, using a cross-linking molecule. This proExM strategy can be used to perform nanoscale imaging of immunostained cells and tissues as well as samples expressing various FPs as fluorescent signals from genetically encoded fluorescent proteins and/or conventional fluorescently labeled secondary antibodies and streptavidin that are directly anchored to the gel are preserved even when subjected to the nonspecific proteolytic digestion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.