Patent · US Active

Three-dimensional human stem cell-derived cortical spheroid model

US10323229B1 · kind B1 · utility

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6Claims
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Key dates

Filing dateNov 14, 2016
Grant dateJun 18, 2019
Priority date
Expiry dateNov 14, 2036

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2510/00
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The present invention provides a new therapeutic method for modeling neuronal responses. Human induced pluripotent stem cells (hiPSCs) emerge recently as alternative sources of primary brain cells to establish Alzheimer's disease (AD) models in vitro. While previous investigations demonstrate the potential of hiPSCs in modeling AD, the 2-D cultures used in these studies cannot fully recapitulate AD-associated neuropathology. 3-D cortical spheroids (forebrain-like structure) were derived from human induced pluripotent stem cells in a bioreactor culture, which can be used to recapitulate AD-associated neuropathology, to screen the therapeutic drugs and to predict neurotoxicity.

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