Biomimetic vascular network and devices using the same

US10327885B2 · kind B2 · utility

Assignees

• The General Hospital Corporation · US
• The Charles Stark Draper Laboratory, Inc. · US

Inventors

• David M. Hoganson · West Chester, US
• Howard I. Pryor · Washington, US
• Ira Spool · Newton, US
• Joseph P. Vacanti · Winchester, US
• Jeffrey T. Borenstein · Holliston, US

Key dates

• Filing date: Feb 6, 2015
• Grant date: Jun 25, 2019
• Expiry date: Feb 6, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5008
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.