High-speed DNA sequencing with optically active nanopore

US10330631B2 · kind B2 · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• Robert H. Blick · Madison, US
• Paul Gwozdz · Hamburg, DE
• Abhishek Bhat · Weilersville, US
• Wolfgang Hansen · Hamburg, DE
• Christian Heyn · Lüneburg, DE

Key dates

• Filing date: Jul 31, 2015
• Grant date: Jun 25, 2019
• Expiry date: Feb 13, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/8827
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A nanoscale-sized pore positioned between two reservoirs may sequence biomolecules by detecting changes in the emitted light due to a change in charge of portions of the biomolecules as they pass through the pore such as affect an emission frequency of a quantum structure proximate to the pore opening. The nanopores may be fabricated using local droplet etching whose randomness is accommodated by lowering the droplet density to permit isolation of nanopores in tiles that may be adhered to an underlying supporting substrate having an aligned opening. The nanopore-tiles may be integrated with commonly applied glass chips and may be employed in microfluidic circuitry.