Patent · US Active

Device and method for large volume transfection

US10336996B2 · kind B2 · utility

2Cited by

3References

12Claims

0Family size

Assignee

Lonza Cologne GmbH · DE

Inventors

Ludger Altrogge · Pulheim, DE
Timo Gleissner · Euskirchen, DE
Andreas Heinze
Sven Hermsmeier · Bonn, DE

Key dates

Filing date	Apr 28, 2015
Grant date	Jul 2, 2019
Priority date	—
Expiry date	Apr 28, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/87
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.