Method and system for characterizing particles using a flow cytometer

US10337975B2 · kind B2 · utility

Assignees

• DEUTSCHES RHEUMA-FORSCHUNGSZENTRUM BERLIN · DE
• A.P.E Angewandte Physik & Elektronik GmbH · DE

Inventors

• Kristen Feher · Berlin, DE
• Toralf Kaiser · Birkenwerder, DE
• Konrad von Volkmann · Berlin, DE
• Sebastian Wolf · London, GB

Key dates

• Filing date: May 5, 2017
• Grant date: Jul 2, 2019
• Expiry date: May 5, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1402
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method and system for characterizing particles using a flow cytometer comprising generating a waveform, as a digital representation of detected radiated light, and transforming said waveform using one or more basis functions and obtaining one or more coefficients characterizing the waveform. The one or more coefficients characterizing the waveform preferably correspond to particular properties of the particle(s), thereby enabling analysis of physical properties of the particles (such as size or shape) or biological properties of the particles, such as cell type, localization and/or distribution of molecules within the cell and/or on the cell surface, structural elements of the cell such as the nucleus or the cytoskeleton, antibody or antibody-fragment binding to the cell or cell morphology. Preferred embodiments of the invention relate to methods and systems in which the waveform is transformed by a wavelet transformation or Fourier transformation.