Assembly activating protein (AAP) and its use for the manufacture of parvovirus particles essentially consisting of VP3

US10344057B2 · kind B2 · utility

Assignees

• Deutsches Krebsforschungszentrum · DE
• Medi&Gene Inc. · DE

Inventors

• Florian Sonntag · Heidelberg, DE
• Juergen Kleinschmidt · Oettingen, DE
• Markus Hoerer · Planegg, DE
• Kerstin Pino Tossi · Munich, DE

Key dates

• Filing date: Sep 12, 2016
• Grant date: Jul 9, 2019
• Expiry date: Sep 12, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14143
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to nucleic acids encoding the novel parvoviral protein “assembly activating protein” (AAP), the encoded polypeptides, methods of producing the polypeptides, antibodies specific for AAP, the use of the nucleic acids for the preparation of the polypeptides, the use of the nucleic acids or the polypeptides for the preparation of the parvoviral particle and methods of producing parvoviral particles essentially consisting of VP3 by providing in addition to the coding sequence of the parvoviral structural protein VP3 a sequence fragment Z/a nucleic acid encoding AAP in the cell and expressing VP3 and fragment Z under control of a rep-independent promoter. Furthermore, the present invention relates to parvoviral particles essentially consisting of VP3 and/oror obtainable by the above method as well as expression cassettes comprising (i) a heterologous promoter and (ii) VP3 coding sequence and/oror fragment Z. The present invention further relates to a medicament, particularly a vaccine, comprising the parvoviral particles or expression cassettes and their use.