Method and a sequence combination for producing nucleic acid fragments
US10344317B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 13, 2014 |
| Grant date | Jul 9, 2019 |
| Priority date | — |
| Expiry date | Feb 23, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2535/122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5′-end extension primer, wherein the single-stranded 5′-end extension primer comprises a sequencing platform adaptor sequence of a 5′ end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3′-end adaptor sequence to the 3′ end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.