DNA sequencing from high density DNA arrays using asynchronous reactions
US10351909B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 2017 |
| Grant date | Jul 16, 2019 |
| Priority date | — |
| Expiry date | Feb 25, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/882
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.