Patent · US Active

Glycoform detection method and glycoform detection device

US10352927B2 · kind B2 · utility

0Cited by
1References
13Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 9, 2013
Grant dateJul 16, 2019
Priority date
Expiry dateAug 27, 2035

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2496/00
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

It is intended to develop and provide a method for detecting a particular glycan-isoform rapidly and specifically by a small number of steps. The present invention provides a glycan-isoform detection method comprising quantifying an immune complex formed by the mixing of a test sample with a sugar chain non-reducing terminal residue-binding lectin and an antibody specifically binding to the protein moiety of the glycan-isoform, etc., comparing the obtained amount of the immune complex with the amount of a control immune complex obtained when a control sample is not mixed with the sugar chain non-reducing terminal residue-binding lectin or is mixed with a control protein, and determining the presence or absence of the glycan-isoform of interest in the test sample on the basis of the difference between these amounts.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.