Process for forming microbubbles with high oxygen content and uses thereof

US10357450B2 · kind B2 · utility

Assignee

• Children's Medical Center Corporation · US

Inventors

• John Kheir · Boston, US
• Lindsay Thomson · Cambridge, US
• Andrew Loxley · Philadelphia, US
• Robert Lee · London, GB

Key dates

• Filing date: Mar 12, 2013
• Grant date: Jul 23, 2019
• Expiry date: Mar 12, 2033

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01F27/271
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Formulations containing a carrier and microbubbles encapsulating oxygen gas, and methods for making and using the formulations are described herein. The formulations are manufactured by a process which includes high shear homogenization. The resulting microbubble suspension may be centrifuged to further concentrate the microbubbles. The resulting concentrated LOM suspension preferably has an oxygen content ranging from 50 to 99% (vol). Prior to administration to a patient, the viscosity of the LOM suspension may be reduced to the desired viscosity, preferably similar to the viscosity of the patient's blood. The resulting LOM formulation typically has an oxygen concentration ranging from 65 to 80% (vol). The microbubbles are formed from one or more lipids, preferably one or more phospholipids, most preferably DSPC, and preferably also contain one or more stabilizing agents/excipients, preferably cholesterol.