Recombinant polymerases with increased phototolerance

US10358634B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Satwik Kamtekar · Mountain View, US
• Lei Jia · Palo Alto, US
• Walter Lee · Campbell, US
• Erik Miller · Seattle, US
• Pranav Patel · Palo Alto, US

Key dates

• Filing date: Aug 4, 2017
• Grant date: Jul 23, 2019
• Expiry date: Jan 4, 2038

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.