Lipase variants and polynucleotides encoding same

US10358635B2 · kind B2 · utility

Assignee

• Novozymes A/S · DK

Inventors

• Padmavathi Balumuri · Chennai, IN
• Rakhi Saikia · Bengaluru, IN
• Allan Svendsen · Paseo Los Muchachos, DK
• Lone Baunsgaard · Elsinore, DK

Key dates

• Filing date: Jun 20, 2018
• Grant date: Jul 23, 2019
• Expiry date: Jun 20, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/01003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.