Factor X mutants

US10364424B2 · kind B2 · utility

Assignee

• Laboratoire Francais du Fractionnement et des Biotechnologies · FR

Inventor

• Jean-Luc Plantier · Val-de-Reuil, FR

Key dates

• Filing date: Feb 3, 2014
• Grant date: Jul 30, 2019
• Expiry date: Jun 2, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/21006
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention concerns a protein comprising a mutated sequence of SEQ ID No. 1, said mutated sequence of SEQ ID No. 1 comprising at least one mutation A, A′, B, C or C′, in which: mutation A consists of substituting amino acids 43 to 52 of sequence SEQ ID No. 1 with a sequence chosen from DFLAEGLTPR (SEQ ID NO: 159), KATN*ATLSPR (SEQ ID NO: 160) and KATXATLSPR (SEQ ID NO: 161), mutation A′ consists of substituting amino acids 47 to 52 of sequence SEQ ID No. 1 with a sequence chosen from TSKLTR (SEQ ID NO: 162), FNDFTR (SEQ ID NO: 163), LSSMTR (SEQ ID NO: 164), PPSLTR (SEQ ID NO: 165) and LSCGQR (SEQ ID NO: 166), mutation B consists of inserting a sequence chosen from DFLAEGLTPR (SEQ ID NO: 159), KATN*ATLSPR (SEQ ID NO: 160), KATXATLSPR (SEQ ID NO: 161), TSKLTR (SEQ ID NO: 162), FNDFTR (SEQ ID NO: 163), LSSMTR (SEQ ID NO: 164), PPSLTR (SEQ ID NO: 165) and LSCGQR (SEQ ID NO: 166), between amino acids 52 and 53 of sequence SEQ ID No. 1, mutation C consists of inserting a sequence chosen from DFLAEGLTPR (SEQ ID NO: 159), KATN*ATLSPR (SEQ ID NO: 160) and KATXATLSPR (SEQ ID NO: 161), between amino acids 52 and 53 of sequence SEQ ID No. 1, and of deleting amino acids 4 to 13 from …