Patent · US Active

Multifunctional protein molecular weight ladders

US10365290B2 · kind B2 · utility

0Cited by
8References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 19, 2017
Grant dateJul 30, 2019
Priority date
Expiry dateJul 28, 2037

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2550/00
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.