Method and microscope for imaging a volume sample

US10365466B2 · kind B2 · utility

Assignee

• LEICA MICROSYSTEMS CMS GMBH · DE

Inventors

• Christian Schumann · Gießen, DE
• Andrea Muelter · Heidelberg, DE

Key dates

• Filing date: Feb 9, 2015
• Grant date: Jul 30, 2019
• Expiry date: Nov 16, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B27/0068
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for microscopically imaging a volume sample includes focusing a microscope objective having a correcting element successively in at least two reference planes which are located within the volume sample at different volume sample depths along the optical axis of the microscope objective; determining, for each reference plane, a reference setting of the correcting element in which an imaging error which is dependent upon the volume sample depth is corrected by the correcting element; determining, on the basis of the reference settings determined for at least one target plane in the volume sample, a target setting for the correcting element in which the imaging error occurring at the volume sample depth of the target plane is corrected by the correcting element; and focusing the microscope objective on the target plane and bringing the correcting element into the target setting in order to image the volume sample.