Non-diffusive botulinum toxin causing local muscle paralysis, and purification method thereof
US10369235B2 · kind B2 · utility
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21References
3Claims
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Key dates
| Filing date | Feb 1, 2017 |
| Grant date | Aug 6, 2019 |
| Priority date | — |
| Expiry date | Feb 1, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/96
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method for purifying a non-spreading botulinum toxin that causes local muscle paralysis and a non-spreading botulinum toxin obtained thereby. The method comprises the steps of: subjecting a purified botulinum toxin type A product to ion-exchange chromatography using a controlled pH of buffer, concentration of sodium chloride (NaCl), thereby separating the botulinum toxin type A product into subfractions; and collecting a subfraction having an A260/A280 value in a specific range from the separated subfractions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.