Method and apparatus for cell isolation, growth, replication, manipulation, and analysis
US10370630B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 2015 |
| Grant date | Aug 6, 2019 |
| Priority date | — |
| Expiry date | Feb 28, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/049
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
According to an aspect of some embodiments, an apparatus for isolation and cytometric analysis of cells from a liquid medium is provided. The apparatus comprises a cell reservoir, for receiving a liquid medium containing a suspension of cells. The apparatus further comprises a cell cage array between the cell reservoir and an enclosed reservoir, each cell cage comprising a large opening adjacent to the first cell reservoir, a cell cage, and one or more small opening adjacent to the enclosed reservoir. A connected liquid medium pump may move the suspended cells into cell cages by flowing the liquid medium from the cell reservoir to the enclosed reservoir. The apparatus further comprises a mechanical element which when actuated, pushes the cell cage array against a gel layer, forming a contiguous barrier to isolate each cell in a cell cage, and allowing cytometric analysis of isolated cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.