Patent · US Active

System for hydrogen production under limited aerobic conditions

US10378028B2 · kind B2 · utility

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9Claims
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Key dates

Filing dateMar 5, 2018
Grant dateAug 13, 2019
Priority date
Expiry dateMar 5, 2038

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12R2001/385
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, E. harbinense YUAN-3, and a facultative anaerobic bacterium, P. aeruginosa PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth of E. harbinense YUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.