Method and system for multiplexed time-resolved fluorescence detection
US10379046B2 · kind B2 · utility
3Cited by
3References
21Claims
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Key dates
| Filing date | Apr 8, 2015 |
| Grant date | Aug 13, 2019 |
| Priority date | — |
| Expiry date | Sep 16, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0245
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a novel method to multiplex long lifetime fluorescent dyes using time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission is used to enhance the ability to separate signals in an assay from multiple dyes. Multiplexed TRF detection apparatuses and systems configured for performing all or part of any of the methods disclosed herein are also provided, particularly apparatuses and systems incorporating cartridge-based multi-mode readers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.