Device and method for single cell screening based on inter-cellular communication
US10385306B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 2015 |
| Grant date | Aug 20, 2019 |
| Priority date | — |
| Expiry date | Dec 18, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54366
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A device for single-cell analysis according to an embodiment of the present invention comprises: a substrate; a gap between the substrate and porous membrane which is a space for culture medium; and a porous membrane formed on having a pore capable of isolating a second cell into single cell units.A method for single-cell analysis according to an embodiment of the present invention comprises: Culturing a first cell in a culture medium on a bottom side of porous membrane; Applying a sample including a second cell on a porous membrane in a culture medium; Isolating the second cell into single cell units in a pore existing in the porous membrane with a external force such as agitation and gravitational force; Generating an interaction situation between the first cells and the single cell-level second cell; Analyzing a cellular phenomena of the first cell or the second cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.